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Organogenesis from leaf and internode explants of Ophiorrhiza prostrata, an anticancer drug (camptothecin) producing plant Electron. J. Biotechnol.
Shahanaz Beegum,A; Poulose Martin,Kottackal; Zhang,Chun-Lai; Nishitha,I. K.; Ligimol,; Slater,Adrian; Madhusoodanan,P. V.
Camptothecin (CPT) analogues and derivatives serve as a novel class of effective anticancer agents that exert their action against DNA topoisomerase I. This paper presents procedures for the rapid, high frequency regeneration of a camptothecin producing plant, Ophiorrhiza prostrata D. Don from leaf and internode explants via shoot organogenesis. The concentrations of plant growth regulators and explant types exhibited discrete roles in the efficacy of shoot induction. N6-benzyladenine (BA) was the most effective cytokinin for the induction of shoots. Murashige and Skoog (MS) medium with 8.87 µM BA and 2.46 µM indole-3-butyric acid (IBA) yielded the highest number of shoots from leaf and internode explants (76.0 and 90.8 shoots respectively). In the case of...
Tipo: Journal article Palavras-chave: Camptothecin; Medicinal plant; Ophiorrhiza prostrata; Plant growth regulators.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100011
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A simple method for isolation of genomic DNA from fresh and dry leaves of Terminalia arjuna (Roxb.) Wight and Arnot Electron. J. Biotechnol.
Deshmukh,Vishal P; Thakare,Prashant V; Chaudhari,Uddhav S; Gawande,Prashant A.
Current protocols for isolation of genomic DNA from Terminalia arjuna have their own limitations due to the presence of high content of gummy polysaccharides and polyphenols. DNA isolated by these protocols is contaminated with a yellowish, sticky and viscous matrix. In our modified DNA isolation method polysaccharides and polyphenols are removed prior to the precipitation of the DNA. Then the genomic DNA was precipitated using isopropanol. This protocol yielded a high molecular weight DNA isolated from fresh as well as dry leaves of T. arjuna, which was free from contamination and colour. Isolated DNA can be used for restriction digestion and PCR amplification. The main objective of the present protocol is to provide a simple method of isolation of DNA,...
Tipo: Journal article Palavras-chave: Ayurveda; DNA extraction; Medicinal plant; Terminalia species.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300014
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Absence of mutagenicity in somatic and germ cells of mice submitted to subchronic treatment with an extract of Croton cajucara Benth. (Euphorbiaceae) Genet. Mol. Biol.
Santos,Fabio V.; Mesquita,Suzana F.P.; Faria,Maria José S.S.; Poersh,Aline; Maciel,Maria Aparecida M.; Pinto,Angelo C.; Morimoto,Helena K.; Cólus,Ilce Mara S..
The plant Croton cajucara Benth. (Euphorbiaceae) is a medicinal plant from the Brazilian Amazon where it is commonly known as sacaca. The principal compound isolated from C. cajucara stem-bark extracts is the clerodane-type diterpene trans-dehydrocrotonin (DCTN) which presents several biological activities, including antiulcerogenic, anti-inflammatory, hypoglycemic, antimutagenic and antitumoral activity. However, few studies have been carried out to evaluate the therapeutic potential of raw C. cajucara extracts. We studied mutagenicity and antimutagenicity effects of C. cajucara methanol extract using the micronucleus assay in bone marrow cells and the dominant lethal assay in mice submitted to subchronic treatments. The blood testosterone levels of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimutagenicity; Mutagenicity; Dominant lethal; Micronucleus; Croton cajucara; Medicinal plant.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100029
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Genotoxic potential of the latex from cotton-leaf physicnut (Jatropha gossypiifolia L.) Genet. Mol. Biol.
de Almeida,Pedro Marcos; de Sousa Araújo,Silvany; Marin-Morales,Maria Aparecida; Benko-Iseppon,Ana Maria; Brasileiro-Vidal,Ana Christina.
Jatropha gossypiifolia L. (Euphorbiaceae), popularly known as cotton-leaf physicnut, is a milky shrub notable for its medicinal properties. The present study aimed to evaluate the toxic, cytotoxic and genotoxic effects of the latex of J. gossypiifolia, using Allium cepa L. as test system. Seeds of A. cepa were exposed to five concentrations of the latex (1.25; 2.5; 5; 10 and 20 mL/L) in order to evaluate parameters of toxicity (evaluation of root growth), cytotoxicity (mitotic index frequency) and genotoxicity (frequency of chromosome alterations). The latex showed a significant decrease in root mean growth value as well as mitotic index for the tested concentrations, except for 1.25 mL/L, when compared to results from the negative control. The 1.25, 2.5...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allium cepa test; Medicinal plant; Plant latex; Genotoxicity evaluation.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100093
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Crude alkaloid extract of Rhazya stricta inhibits cell growth and sensitizes human lung cancer cells to cisplatin through induction of apoptosis Genet. Mol. Biol.
Elkady,Ayman I..
There is an urgent need to improve the clinical management of non-small cell lung cancer (NSCLC), one of the most frequent causes of cancer-related deaths in men and women worldwide. Rhazya stricta, an important medicinal plant used in traditional Oriental medicine, possesses anti-oxidant, anti-carcinogenic and free radical scavenging properties. This study was done to explore the potential anticancer activity of a crude alkaloid extract of R. stricta (CAERS) against the NSCLC line A549. CAERS markedly suppressed the growth of A549 cells and considerably enhanced the anti-proliferative potential of cisplatin. CAERS-mediated inhibition of A549 cell growth correlated with the induction of apoptosis that was accompanied by numerous morphological changes, DNA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apoptosis; Lung cancer; Medicinal plant; RT-PCR; Western blot.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100003
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In vitro establishment of pepper-rosmarin nodal segments Horticultura Brasileira
Blank,Arie F; Costa,Andréa S da; Arrigoni-Blank,Maria de Fátima; Mendonça,Aline B de; Ledo,Ana da S.
Pepper-rosmarin (Lippia sidoides Cham.) is a native medicinal plant from the Brazilian biome Caatinga. Its high economical importance comes from the antimicrobial properties of thymol and carvacrol, both present in the pepper-rosmarin essential oil. Nodal segments of pepper-rosmarin were established in vitro to evaluate different growth regulators. We tested four concentrations of IBA (0.0; 0.01; 0.05; and 0.1 mg L-1) combined with six concentrations of BAP (0.0; 0.1; 0.5; 1.0; 2.0; and 4.0 mg L-1) in assay 1; five concentrations of NAA (0.0; 0.1; 0.25; 0.5; and 1.0 mg L-1) in assay 2; two concentrations of NAA (0.0 and 0.01 mg L-1) combined with five concentrations of kinetin (0.0; 0.25; 0.5; 1.0; and 2.0 mg L-1) in assay 3; five concentrations of IAA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lippia sidoides; Medicinal plant; Micropropagation; Growth regulator.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362008000200025
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Growth analysis of Fafia in relation to the propagation method used Horticultura Brasileira
May,André; Bovi,Odair A; Maia,Nilson B; Moraes,Andrea RA de; Pinheiro,Mariane Q.
A trial was carried out in Campinas, Brazil, from August 2005 to August 2006, in order to analyze the effect of two propagation methods (seeds and cuttings) on the development of Pfaffia glomerata (Spreng.) Pedersen. The experimental design was a completely randomized block, with five replications, and two methods of plant propagation. Plant height, aerial part dry mass and root dry mass were evaluated. Plants propagated from cuttings showed greater accumulation of dry mass on the aerial part. Root dry mass accumulation curves showed an exponential pattern, and at the end of the experimental period, the average of roots dry mass for both treatments were similar, up to 349,65 g plant-1.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pfaffia glomerata; Brazilian ginseng; Medicinal plant; Propagation.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362008000300016
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Harvest time and plant age on the content and chemical composition of the essential oil of Alpinia zerumbet Horticultura Brasileira
Santos,Martiely S; Jezler,Caroline N; Oliveira,Ariana RMF de; Oliveira,Rosilene A; Mielke,Marcelo S; Costa,Larissa CB.
Alpinia zerumbet is an aromatic and medicinal plant rich in essential oil, known as colônia. Essential oils are derived from secondary metabolism and may be a source of raw materials for cosmetic, pharmaceutical, food and perfumery industry. The plant secondary metabolism and biosynthetic activity can vary according to endogenous and exogenous factors to which it is exposed. In this context, in this study we evaluated the influence of harvest time and plant age of Alpinia zerumbet on biomass and essential oil production. For the harvest time experiment the plants of A. zerumbet were harvested at different times (8, 10, 12, 14, 16 and 18 h), using a completely randomized design with four replications. In the plant age experiment the seedlings were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zingiberaceae; 'colônia'; Medicinal plant; GC analysis; Terpinen-4-ol.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362012000300005
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Biomass production and essential oil of lemon balm cultivated under colored screens and nitrogen Horticultura Brasileira
Alves,Aglair C; Jesus,Fábio N; Alves,Péricles B; Santos,Hamilton V; Souza,Girlene S; Santos,Anacleto R.
ABSTRACT The aim of this study was to evaluate the effect of light quality and nitrogen availability on the biomass production, yield and composition of essential oil of lemon balm (Lippia alba). Five doses of N (0; 40; 80; 120 and 160 kg ha-1) were studied, using urea and three light quality conditions obtained with the use of colored shading nets (blue and red), 50% irradiance and under full sun. The experiment was arranged in a 5x3 factorial scheme, in split plots, with seven replicates. After 60 days of cultivation under the shading nets, a higher dry leaf biomass (4.83 g per plant) was observed in the plants under blue net cultivation at a dose of 102 kg ha-1 of N. The plants grown under full sun presented greater amount of neral and geranial, major...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lippia alba; Shading nets; Nitrogen fertilization; Medicinal plant; Light quality.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000100094
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Nitrogen sources on yield, mineral nutrition and bromatology of Cyclanthera pedate Horticultura Brasileira
Valadares,Samuel V; Valadares,Rafael V; Costa,Candido A; Martins,Ernane R; Fernandes,Luiz A.
ABSTRACT Caygua fruits (Cyclanthera pedata) are used both in cooking and for medicinal purposes. However, few studies on this species, considered a non-conventional vegetable, can be found in literature. This study aimed to assess the responses of caygua crop to green manure and mineral nitrogen fertilization, in relation to marketable fruit productivity, nutrient content in leaves and fruits and bromatological composition. The study was carried out in a randomized block design with four treatments and five replicates: 1) control (without nitrogen); 2) 60 kg ha-1 nitrogen (urea form); 3) green manuring using Cajanus cajans and 4) green manuring using Crotalaria juncea. The plots consisted of three caygua lines with four plants per line (spacing 2x1 m). In...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cyclanthera pedata; Green manuring; Non-conventional vegetable; Medicinal plant.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362020000100078
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Genetic diversity among natural populations of Mandevilla velutina Horticultura Brasileira
Bertoni,Bianca Waléria; Souza,Ana Valéria de; Biondo,Ronaldo; França,Suzelei de C; Telles,Mariana PC; Pereira,Ana Maria S.
Mandevilla velutina (Mart. Ex Stadelm) Woodson (Apocynaceae) is an endemic species in the Cerrado (Brazilian Savannah), whose alcoholic extracts and root infusions are extensively used in the popular medicine to treat inflammatory diseases and against snake bites. Currently, this species has been pointed out as crucial in conservation programs. Therefore, studies on its genetic diversity, as well as the development of methodology for in vitro conservation in germplasm banks are imperative to avoid its extinction. The objective of this work was to investigate the intra- and inter-populational genetic variability of M. velutina to enhance the representativeness of germplasm banks. To this aim, we used RAPD molecular markers. The analysis of molecular...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mandevilla velutina; Medicinal plant; Extinction risk; Molecular markers.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362010000200012
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Levantamento de plantas nativas da caatinga usadas em tratamentos de animais domésticos. Infoteca-e
TORRES, D. M.; INACIO NETO, A..
Avaliação do efeito de plantas nativas da caatinga usadas pela população da região para tratamento de doenças dos animais domésticos. O questionário foi aplicado em diversas propriedades agrícolas e feiras livres.
Tipo: Comunicado Técnico (INFOTECA-E) Palavras-chave: Domestic animal; Disease; Medicinal plant; Verminosis; Treatment.; Caatinga; Animal Domestico; Planta Medicinal; Tratamento; Verminose..
Ano: 1993 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/133600
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Ethnomedicinal plants used for snakebite treatments in Ethiopia: a comprehensive overview J. Venom. Anim. Toxins incl. Trop. Dis.
Yirgu,Abraham; Chippaux,Jean-Philippe.
Abstract Traditional medicine plays an important role in the daily lives of people living in rural parts of Ethiopia. Despite the fact that Ethiopia has a long history of using traditional medicinal plants as an alternative medicine source, there is no checklist compiling these plants used for snakebite treatment. This review collected and compiled available knowledge on and practical usage of such plants in the country. A literature review on medicinal plants used to treat snakebites was conducted from 67 journal articles, PhD dissertation and MSc theses available online. Data that summarize scientific and folk names, administration methods, plant portion used for treatment and method of preparation of recipes were organized and analyzed based on citation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ethnobotany; Medicinal plant; Traditional treatment; Snakebite; Envenomation; Sub-Saharan Africa; Ethiopia.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100205
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Ternstroemia cameroonensis (Ternstroemiaceae), a new medicinally important species of montane tree, nearly extinct in the Highlands of Cameroon Naturalis
Cheek, M.; Tchiengue, B.; Tacham, W.N..
Ternstroemia cameroonensis sp. nov. (Ternstroemiaceae) is formally described from the Highlands of Cameroon and its conservation status is assessed as Critically Endangered using the IUCN 2012 standard. This is the third species of Ternstroemia known from Africa. The species is unusual in its genus in being monoecious, having Terminalia-branching (Aubreville’s model), phyllotaxy of 1/3 (not pseudo-verticillate), flowers with a uniseriate androecium and a distinct intra-staminal disc, and in the stamens lacking a distinct apicular connective. The species is used locally for extensive medicinal purposes.
Tipo: Article / Letter to the editor Palavras-chave: Cloud Forest; Critically Endangered; Medicinal plant; Pentaphylacaceae; Theaceae.
Ano: 2017 URL: http://www.repository.naturalis.nl/record/626072
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Water stress before harvest of pepper-rosmarin PAB
Alvarenga,Ivan Caldeira Almeida; Valadares,Rafael Vasconcelos; Martins,Ernane Ronie; Oliveira,Flávio Gonçalves; Figueiredo,Lourdes Silva de; Kobayashi,Mauro Koji.
The objective of this work was to assess the effect of different periods of water stress before harvest of pepper-rosmarin (Lippia sidoides) on the contents of essential oil and flavonoids. The experiment was carried out during 270 days of cultivation, with drainage lysimeters, in a completely randomized block design with five treatments: 0, 2, 4, 6, and 8 days of water suppression before harvest, with four replicates. Fresh and dry matter yield, essential oil content, total flavonoids content, and water potential and temperature of leaves were determined. There was a decrease of approximately 50% in oil content and of 60% in total flavonoid content with the reduction of leaf water potential in 0.3 MPa. Essential oil is more sensitive to water stress than...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lippia sidoides; Essential oil; Flavonoids; Leaf water potential; Medicinal plant; Water stress.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000700005
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Yield and Composition of the Essential oil of Tetradenia riparia (Hochst) Codd (Lamiaceae) Cultivated Under Different Shading Levels Planta Daninha
ARAÚJO,L.L.N.; MELO,H.C.; PAULA,J.R.; ALVES,F.R.R.; PORTES,T.A..
ABSTRACT: Light has direct influence on growth and development by altering the morphophysiology of the plant and the content and composition of secondary metabolites. The present study aimed to evaluate the yield and composition of the essential oil of Tetradenia riparia cultivated under different shading levels. Plants were propagated by cuttings and cultivated either under full sunlight and in environment completely covered by black polyethylene sheeting to obtain shading levels of 30%, 50%, and 80%. Plants were grown under experimental conditions for 150 days. The essential oil was extracted from fresh leaves in triplicate for each treatment through steam distillation with a modified Clevenger apparatus. The components of the essential oil were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 14-hydroxy-9-epi-(E)-caryophyllene; Medicinal plant; Secondary metabolites; Shading levels.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582018000100266
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Intermittent drying of clove basil leaves: Process optimization and essential oil yield AGRIAMBI
Souza,Raiane A. de; Melo,Evandro de C.; Ávila,Mariane B. R. de; Gonzaga,Diego A.; Sperotto,Naiara C. Z.; Carneiro,Antônio P. S..
ABSTRACT This study investigated the effect of a drying method with intermittent ratios on the effective drying time, specific energy consumption and essential oil yield of clove basil (Ocimum gratissimum L.) leaves. The drying tests were conducted at the Postharvest Laboratory of Medicinal, Aromatic and Condiment Plants, from the Departamento de Engenharia Agrícola of the Universidade Federal de Viçosa, Viçosa, MG, Brazil (20° 46’ 13.5” S; 42° 52’ 23.3” W, and altitude of 645 m) in October and November 2017. The experiment was conducted by using a 3 × 4 factorial scheme including three drying temperatures at 50, 60 and 70 °C, with four drying processes: continuous drying and the following three intermittent drying/tempering ratios in minutes: 10/20, 15/15...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ocimum gratissimum L.; Medicinal plant; Tempering time; Effective drying time; Specific energy consumption.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662020000300209
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Essential oil production of lemongrass (Cymbopogon citratus) under organic compost containing sewage sludge AGRIAMBI
d'Ávila,Júlia V.; Martinazzo,Ana P.; Santos,Fabiana S. dos; Teodoro,Carlos E. de S.; Portz,Adriano.
ABSTRACT One of the main urban polluting agents are the sewers, which even with proper treatment end up generating a polluting waste, the sewage sludge. One of the options for the disposal of this sludge is the use in agriculture, due to its high content of organic matter and nutrients. This study aimed to use urban sewage sludge for lemongrass cultivation and essential oil production. The plants were grown in soil containing different organic compost doses (0, 5, 10, 20, 40 and 60 t ha-1), formed from the sewage sludge composting process and waste of urban vegetation pruning. At harvest, plants were analyzed for the concentration of nutrients, chlorophyll content, number of tillers, biomass production, essential oil content and the microbiological quality...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solid waste; Medicinal plant; Composting; Mineral nutrition.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662016000900811
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Drying kinetics of blackberry leaves AGRIAMBI
Martins,Elton A. S.; Goneli,André L. D.; Goncalves,Alexandre A.; Hartmann Filho,Cesar P.; Siqueira,Valdiney C.; Oba,Guilherme C..
ABSTRACT Blackberry leaves have some pharmacological properties and one of the most widespread and studied uses is to relieve symptoms of the climacteric and other symptoms during the premenstrual period. Thus, drying becomes important for the conservation and storage of the product until its use or processing. The present study aimed to evaluate the drying kinetics of blackberry leaves, as well as to determine the effective diffusion coefficient and the activation energy during the drying process. Blackberry leaves were dried in an experimental fixed-bed dryer under four controlled temperature conditions (40, 50, 60 and 70 °C) and two drying air speeds (0.4 and 0.8 m s-1). With the experimental data of moisture ratio, eight mathematical models were fitted...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Morus nigra L.; Medicinal plant; Air speeds; Midilli model.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662018000800570
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Influence of photoperiod on the accumulation of allantoin in comfrey plants Rev. Bras. Fisiol. Veg.
CASTRO,ANA HORTÊNCIA FONSÊCA; YOUNG,MARIA CLÁUDIA MARX; ALVARENGA,AMAURI ALVES DE; ALVES,JOSÉ DONIZETI.
In order to evaluate the influence of photoperiod on the accumulation of allantoin in comfrey (Symphytum officinale L.), a well known medicinal plant, an experiment was conducted during July and August, 1998. Cuttings obtained from 90 day old plants were submitted to four photoperiods (8, 12, 16 and 20 hours). After 60 days, allantoin content in roots and rhizomes was evaluated. The results showed that increases in photoperiod promoted an increment in the average content of allantoin in roots (0.06%, 0.303%, 1.213% and 4.78%). On the other hand, in rhizomes, allantoin accumulation decreased (9.65%, 7.14%, 0.55%) when the photoperiod was increased from 8 to 12 and 16 hours, respectively, stabilizing on a 20-hour photoperiod (0.53%). Plants cultivated under...
Tipo: Info:eu-repo/semantics/article Palavras-chave: HPLC; Medicinal plant; Secondary metabolism; Symphytum officinale; Ureides.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-31312001000100006
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